monoclonal anti-human/mouse cd282 tlr2 purified antibody (clones t2.5 and 6c2)  (Thermo Fisher)

Journal: Applied and Environmental Microbiology

Article Title: Piliation of Lactobacillus rhamnosus GG Promotes Adhesion, Phagocytosis, and Cytokine Modulation in Macrophages

doi: 10.1128/AEM.03949-14

Figure Lengend Snippet: Role of CD11b in adhesion and uptake of L. rhamnosus GG by RAW 264.7 cells and effect of TLR2 in the subsequent immunomodulatory response. (A) Role of CD11b in adhesion of the mutants to macrophages (left) and phagocytosis of the mutants by macrophages (right) relative to the results for the wild-type strain, which were set equal to 1. Bacterial strains (5 × 107 CFU/ml) were coincubated for 1 h with 1 × 106 RAW 264.7 cells that had previously been treated with anti-mouse CD11b monoclonal antibody (5 μg/ml) for 30 min. The data are presented as the mean relative adhesion or phagocytosis ± standard deviation. These experiments were done in triplicate. Asterisks represent statistically significant differences compared to the results for the controls (no antibody treatment). (B) The effect of TLR2 on the relative levels of IL-10 and IL-6 mRNA expression by RAW 264.7 macrophages was analyzed by pretreatment of macrophages with antibodies against TLR2 (5 to 10 μg/ml) for 30 min and then coincubation with L. rhamnosus GG strains for 3 h. Bacteria were administered at a ratio of 1:50 of cells to bacteria. The results are mean values ± standard deviations from three separate experiments. The values are normalized against those for GAPDH. Asterisks denote statistically significant differences from the results for the control (no antibody treatment).

Article Snippet: The blocking monoclonal anti-human/mouse CD282 TLR2 purified antibody (clones T2.5 and 6C2) and monoclonal anti-mouse CD11b purified antibody (clone M1/70) were obtained from eBioscience (San Diego, CA, USA).

Techniques: Standard Deviation, Expressing

Journal: Applied and Environmental Microbiology

Article Title: Piliation of Lactobacillus rhamnosus GG Promotes Adhesion, Phagocytosis, and Cytokine Modulation in Macrophages

doi: 10.1128/AEM.03949-14

Figure Lengend Snippet: Role of CD11b in adhesion and uptake of L. rhamnosus GG by RAW 264.7 cells and effect of TLR2 in the subsequent immunomodulatory response. (A) Role of CD11b in adhesion of the mutants to macrophages (left) and phagocytosis of the mutants by macrophages (right) relative to the results for the wild-type strain, which were set equal to 1. Bacterial strains (5 × 107 CFU/ml) were coincubated for 1 h with 1 × 106 RAW 264.7 cells that had previously been treated with anti-mouse CD11b monoclonal antibody (5 μg/ml) for 30 min. The data are presented as the mean relative adhesion or phagocytosis ± standard deviation. These experiments were done in triplicate. Asterisks represent statistically significant differences compared to the results for the controls (no antibody treatment). (B) The effect of TLR2 on the relative levels of IL-10 and IL-6 mRNA expression by RAW 264.7 macrophages was analyzed by pretreatment of macrophages with antibodies against TLR2 (5 to 10 μg/ml) for 30 min and then coincubation with L. rhamnosus GG strains for 3 h. Bacteria were administered at a ratio of 1:50 of cells to bacteria. The results are mean values ± standard deviations from three separate experiments. The values are normalized against those for GAPDH. Asterisks denote statistically significant differences from the results for the control (no antibody treatment).

Article Snippet: The blocking monoclonal anti-human/mouse CD282 TLR2 purified antibody (clones T2.5 and 6C2) and monoclonal anti-mouse CD11b purified antibody (clone M1/70) were obtained from eBioscience (San Diego, CA, USA).

Techniques: Standard Deviation, Expressing, Bacteria, Control